Sulfonamides Rapid Test Uncut Sheet

Sulfonamides Rapid Test -Aquatic Products

1.        INTENDED USE
Sulfonamides Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Sulfonamides residue in animal’s tissue.
Assay Time:  5 - 10 min

2.        PRINCIPLE OF THE ASSAY
Sulfonamides Rapid Test is based on competitive lateral flow immunochromatographic assay. The SULFA-conjugate in the test zone will capture the immuno-gold (colloid gold-SULFA antibody conjugate), when there is very little dissociative SULFA in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The invisible test band (T zone) means a positive result.

3.        KIT COMPONENT

-       10×foil pouches each containing a cassette, pipette and desiccant

-       12×centrifugal tubes (2×,15 mL; 10×,1.5 mL)

-       2×graduated pipettes (3 mL )

-       Product Manual

4.        TEST PROCEDURE

-       Collect Aquatic Products as sample. Get rid of the fat tissue. Homogenize the sample at 10000 rpm for 1 min. Weigh out 4.0 g of sample into a 15 mL centrifugal tube. Tightly cover the lid.

-       Put the tube into a water bath (80°C ) for 10 min. Take out as much the extract into a 1.5 mL of centrifugal tube.

-       Do centrifugation at 4000 rpm for 1 min to make the extract clear.

-       Take out the cassette from the foil pouch and place it horizontally.

-       Gradually drip 3 drops of sample extraction into the sample hole “S”.

-       Interpret the result in 5 - 10 min. Result after 10 min is only considered as reference. 

5.        INTERPRETATION OF RESULTS

Positive: Only clear C band appears.

Negative: The presence of both C band and T band, no matter T band is clear or vague.

Invalid: No colored band appears in C zone, no matter whether T band appears.

6.        SENSITIVITY AND SPECIFICITY

-       The results are negative when the test is applied to detect 100 ppm of Tetracycline, chlorph, Macrolides, Beta lactam and fluoro-quinolones. 

7.        STORAGE
The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

8.        PRECAUTIONS

-       For best results, please strictly adhere to these instructions.

-       All reagents must be at room temperature before running the assay.

-       Do not remove test cassette from its pouch until immediately before use.

-       Do not reuse the test kit.

-       Do not use the test beyond its expiration date marked on the foil pouch.

-       The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.  

9.        LIMITATION
Sulfonamides Rapid Test is a useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. It provides a semi-quantitative method to detect the Sulfonamides in Aquatic. If you want a quantitative result, please adopt other method such as ELISA in practice.