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Salmonella Rapid Test-Food safety
Salmonella Rapid Test is a sandwich lateral flow immunochromatographic assay for the qualitative detection of Salmonella in food sample.
LOD: 5 X 104 cfu/mL
Assay Time: 5 - 10 min
PRINCIPLE OF THE ASSAY
Salmonella Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is enough Salmonella in the sample, a visible T band will appear. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Salmonella in the sample.
10×foil pouches, each containing one cassette, one pipette and a desiccant
1. Frozen meat, egg products, dairy products, and other processed foods : smash the solid foods or emulsify the powdery foods with glass rod. Measure 25g (25ml) and put it into 500ml wide-mouth bottle with 225ml BP solution. Cultivate 4h at the 36±1℃ (Dry egg products for 18-24h).Then measure 10ml and add into 100ml MM or TTB enrichment broth, cultivate 18-24h at 42℃. Or measure 10ml and subcultivate into 100 ml SC enrichment broth ,cultivate 18-24 h at the 36±1℃ to get the last test solutions.
2.Fresh meat, fresh eggs ,fresh milk or other raw foods: smash the solid foods or emulsify the powdery foods with glass rod. Measure 25g(25ml) and put it into 25ML sterile Normal Saline and mix. Then add into 100ml MM or TTB enrichment broth, Cultivate 24h at 42℃; or measure 25ml and subcultivate into 100 ml SC enrichment broth ,cultivate 18-24 h at the 36±1℃ to get the last test solutions.
P.S: Buffered Peptone Water (BP), Rappaport-Vassiliadis (MM) Enrichment Broth , TTB Enrichment Broth , Selenite Cystine (SC) Enrichment Broth, Bismuth Sulfite Enrichment Broth: According to GB 4789.28
3.Take out the cassette from the foil pouch and place it horizontally.
4.Suck the broth and drip 3 drops into the assay sample hole “S”.
5.Interpret the result after 5 minutes. Result after 10 minutes is considered as invalid.
INTERPRETATION OF RESULTS
Positive: The presence of both C band and T band, no matter T band is clear or vague.
Negative: Only clear C band appears.
Invalid: No colored band appears in C zone, no matter whether T band appears.
The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.
For best results, please strictly adhere to these instructions.
All reagents must be at room temperature before running the assay.
Do not remove test cassette from its pouch until immediately before use.
Do not reuse the test kit.
Do not use the test beyond its expiration date marked on the foil pouch.
The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
Salmonella Rapid Test is for in vitro testing only. For an accurate result, It is suggested to apply other method such as PCR for final determination in practice.
Contact Rebecca Yan
Contact Person: Ms. Anna Lee