HIV 1/2 Saliva Home Use Test

HIV 1/2 Saliva Home Use Test

Arapid ,one step lateral flow immunoassay for the qualitative detection of HIV-1/2 antibodiesin human saliva For in vitrodiagnostic use

INTRODUCTION

The  Saliva  HIV-1/2 Antibody  Screen  Test  is  a  rapid chromatographic  immunoassay  for  the  qualitative  detection  of antibodies  to  Human  Immunodeficiency  Virus  type  1  and  2 (HIV-1/2)  in  human  saliva.  All  positive  specimens  must  be confirmed with Western Blot or other qualified EIA.

PRINCIPLE

It  has  been  shown  that  the  acquired  immunodeficiencysyndrome  (AIDS)  is  caused  by  viruses which transmitted by sexual  contact,  transfusion,  using contaminated  blood  products and  sharing  contaminated  needles.  HIV-1  and  2  viruses  have been  isolated  from  patients  with  AIDS  and  AIDS- related complex  (ARC),  high  risk  persons for  AIDS.  HIV-1/2  viruses deplete  T  helper  cells,  a  subpopulation  of  T  cells  for  body defense, thus causing AIDS patients susceptible to opportunistic infections  and  developing  malignant  tumors.  The  incidence  of specific  antibodies  to  HIV-1/2  is  high  in  AIDS,  ARC  and persons  with  high  risk  for  AIDS.  The    HIV-1/2  Antibody Saliva  Rapid  Screen  Test  was  designed  to  detect  antibodies to HIV- 1/2  in  AIDS  patients,  ARC  or  high  risk  persons  and identify  any  potential  donors  carrying  these  antibodies  in specimens.The Saliva HIV-1/2  Antibody  Screen  Test is a chromatographic immunoassay  for  the  detection  of  antibodies  to  HIV-1/2  in human saliva. HIV - 1/2 antibodies binding protein are precoated onto  membrane  as  capture  reagents  i n  the  Test  region  (T). During  the  testing,  the  specimen  is  allowed  to  react  with  the colloidal  gold  reagents  which  have  been  labeled  with  HIV-1/2 specific  antigens.  Antibodies  to  HIV-1/2,  if  present, binds  to antigens coated on colloidal gold, anda pinkcolored band will develop  in the test region (T) on the membrane.  Absence of this pink colored  band  in  the  test  region  (T)  suggests  a  negative result. To serve as a procedural control, a pin kcolored band in the  control  region  (C)  will  always  appear  regardless  of  the presence or absence of antibodies to HIV-1/2 in human saliva.

Rebecca Yan