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Bovine Foot and Mouth Disease Virus Ab Rapid Test
Bovine Foot and Mouth Disease Virus Ab Rapid Test is a sandwich lateral flow immunochromatographic assay for the qualitative detection of Bovine Foot and mouth disease virus antibody (FMD Ab) in pig’s serum.
Assay Time: 10- 20 min
PRINCIPLE OF THE ASSAY
Bovine Foot and Mouth Disease Virus Ab Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is enough FMD antibodies in the sample, a visible T band will appear. The color intensity of T band has a positive correlation with the titre of FMD Ab. The C band should always appear after a sample is applied, indicating a valid result. With the color card, the device can accurately indicate the titre of Bovine FMD antibodies in the sample.
10×foil pouches, each containing one cassette, one pipette and a desiccant
1.Do centrifugation to Bovine’s whole blood sample to collect serum for use.
2.Take out the cassette from the foil pouch and place it horizontally.
3.Gradually drip 3 drops of serum/plasma into the sample hole “S”.
4.Interpret the result in 10-20 minutes. Result after 20 minutes is only considered as reference.Compare color intensity of T band with color card.
INTERPRETATION OF RESULTS
1.If the color of T band is similar or darker than the one of 1 : 32 on the color card, vaccination is not required.
2.If the color of T band is ligther than the one of 1 : 32 on the color card, vaccination is required.
3.A deeply dark T band means that infection may have occured. Further detection is suggested.
Negative: Only clear C band appears.
Invalid: No colored band appears in C zone, no matter whether T band appears.
The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.
1.For best results, please strictly adhere to these instructions.
2.All reagents must be at room temperature before running the assay.
3.Do not remove test cassette from its pouch until immediately before use.
4.Do not reuse the test kit.
5.Do not use the test beyond its expiration date marked on the foil pouch.
6.The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
Bovine Foot and Mouth Disease Virus Ab Rapid Test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, It is suggested to apply other method such as ELISA for final determination in practice.
Span Biotech Ltd.
Contact Person: Ms. Anna Lee