Beta-agonist ELISA Test Kit, Food Safety

Introduction

Specifications

Principle: Direct enzyme-linked immunosorbent assay for the quantitative detect

Intended use: Detection of Beta-agonist

Specimen: Animal’s tissue, urine and feed.

Incubation Time: 30 min + 15 min

Standards: 0, 0.1, 0.3, 0.9, 2.7, 8.1 ng/mL

Detection Limit: Animal’s tissue 0.4 ppb / 0.2 ppb,Urine 0.1 ppb,Feed 5 ppb

Cross reaction:

Clenbuterol 100%,

Salbutamol 105%,

Tulobuterol 116.5%,

Pirbuterol 150%,

Carbuterol 191.5%,

Terbutaline 46.8%,

Mabuterol 40.1%,

Methyl-Clenbuterol 29.4%,

Cimaterol 6.4%,

Orciprenaline <7.8%

Recovery Rate: 90%±15% (pork liver 70%±15%)

Precision: Intra-assay CV<8% Inter-assay CV<15%

Shelf life: 12 months

Storage: 2- 8 °C. No freezing.

Packing size: 96 wells / kit

Advantages

Beta-agonist ELISA Kit enables the detection of very low residual level.

Beta-agonist ELISA Kit is a quick and accurate testing tool.

Beta-agonist ELISA Kit can be stored for 12 months between 2 and 30°C.

Instructions

Kit Component

- 1 X Microtiter plate (8 microwells X 12 removable strips)

- 6 X Standard solutions (1 mL each): 0 ng/ mL, 0.1 ng/ mL, 0.3 ng/ mL, 0.9 ng/ mL, 2.7 ng/ mL, 8.1 ng/ mL

- 1 X Standard solution (1 mL, for standard substance adding testing): 100 ng/mL

- 1 X Enzyme-linked conjugate (13 mL)

- 1 X Washing buffer concentrate (10 X , 50 mL)

- 1 X Substrate A (6 mL)

- 1 X Substrate B (6 mL)

- 1 X Stopping solution (6 mL)

- 1 X Product Manual

Materials Required But Not Provided

- ELISA Microtiter plate reader equipped with 450 nm filters

- 50 – 200 μL multichannel micropipette

- 50, 100 and 200 μL precision micropipette

- Microplate washer or squeeze bottle

- Homogenizer

- Centrifuge

- Centrifugal tubes

- Distilled water

- HCl, NaOH

Rebecca Yan