Rat Peroxisome Proliferator-activated receptor γ(PPAR-Γ)ELISA Kit

Rat Peroxisome Proliferator-activated receptor γ(PPAR-Γ)ELISA Kit

SAMPLE TYPES VALIDATED

Serum, blood plasma, Saliva, Urine, and other related tissue Liquid.

Please read this insert completely prior to using the product.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Please carefully read this instruction before using. This ELISA kit is based on the principle of double-antibody sandwich technique to detect Rat  Peroxisome Proliferator-activated receptor γ(PPAR-Γ).. Be used only for research purposes, not be used for medical diagnosis.

INTENDED USE

This kit is used to assay the Peroxisome Proliferator-activated receptorγ(PPAR-Γ) in the sample of Rat’s serum, blood plasma, and other related tissue Liquid.

PRINCIPLE

The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Rat Peroxisome Proliferator-activated receptor γ(PPAR-Γ)in samples. Add Peroxisome Proliferator-activated receptor γ(PPAR-Γ) to monoclonal antibody Enzyme well which is pre-coated with Rat Peroxisome Proliferator-activated receptor γ(PPAR-Γ)monoclonal antibody, incubation; then, add Peroxisome Proliferator-activated receptor γ(PPAR-Γ)antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concentration of the Rat Substance Peroxisome Proliferator-activated receptor γ(PPAR-Γ)of sample were positively correlated.

Materials supplied in the Test Kit

Materials required but not supplied

1. 37 ℃ incubator                                                     2. Standard Enzyme reader

3. Precision pipettes and Disposable pipette tips      4. Distilled water

5. Disposable tubes for sample dilution                     6. Absorbent paper

Important Notes

1. Beening taken out from the 2-8℃ environment, the kit should be balanced 30 minutes in the ambient temperature then use. If the Coated plates of Enzyme haven’t been used up after opened, the remaining plates should be stored in Sealed bag.

2. For each step, add Sample with sample injector which should be calibrated frequently, in order to avoid unnecessary experimental tolerance.

3. The operation shall be carried out accordance to the instructions strictly. And test results must be based on the readings of the Enzyme reader.

4. In order to avoid cross-contamination, it is forbidden to re-use the suction head and seal plate membrane in your hands.

5. All samples, washing buffer and each kind of reject should according to infective material process.

6. The idle agents shall be put up or covered. Do not use reagent with different batches. And use them before expired date.

7. The substrate B is light-sensitive. Prolonged exposure to light is forbidden.

Washing method

Manually washing method: shake away the remain liquid in the enzyme plates; place some bibulous papers on the test-bed, and flap the plates on the upside down strongly. Inject at least 0.35ml after-dilution washing solution into the well, and marinate 1~2 minutes. Repeat this process according to your requirements.

Automatic washing method: if there is automatic washing machine, it should only be used in the test when you are quite familiar with its function and performance.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level  Rat PPAR-Γwere tested 20 times on one plate, respectively. 

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rat INS were tested on 3 different plates, 8 replicates in each plate. 

CV(%) = SD/meanX100

Intra-Assay: CV<10% 

Inter-Assay: CV<12%