Human C-Reactive Protein(CRP) ELISA Kit

Human C-Reactive Protein(CRP) ELISA Kit

Package: 96 tests per kit

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Please read this insert completely prior to using the product. This kit is used to assay Human C-Reactive Protein(CRP) on the basis of the Biotin double antibody sandwich technology. This Elisa kit takes one-step method with which solutions do not need diluting,because we simplifies the dilute process by our lab techniques. This kit is for research only and is not for use in diagnostic procedures.

Intended Use

This kit is used to assay the C-Reactive Protein(CRP)in the sample of Human’s serum, blood plasma and other related biological liquid.

Test principle

This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Human C-Reactive Protein (CRP). Add C-Reactive Protein(CRP)to the wells, which are pre-coated with C-Reactive Protein(CRP)monoclonal antibody and then incubate. After that, add anti CRP antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Human C-Reactive Protein (CRP) are positively correlated.

Materials supplied in the Test Kit

Materials required but not supplied

1. 37 ℃ incubator                              2. Standard Enzyme reader

3. Precision pipettes and Disposable pipette tips      4. Distilled water

5. Disposable tubes for sample dilution             6. Absorbent paper

Important Notes

1. Before opening the kit kept at the temperature of 2-8℃, it takes at least 30 minutes to increase naturally to room temperature. After breaking the seal of ELISA coated-plate, some of the stripes used should be kept in hermetic bag.

2.When adding samples, sample injector must be used for each time and should also be frequently checked for its precision to avoid individual error.

3. The instruction must be strictly followed while the reading of ELISA reader must be set as the standard of determining the experiment result.

4.Pipette tips and seal plate membrane in hand should not be used more than once in order to avoid cross contamination.

5.All samples, washing concentration and wastes of every kind should be disposed as infective agent.

6.Other reagents not needed must be packed or covered. Reagents of different batches must not be mixed and should be used before their respective validity dates.

7. Substrate B is sensitive to light and therefore should not be exposed to light for too long.

Washing method

Manually washing method: Washing by hand: Shake off the liquids in the wells of the ELISA plate; Lay several bibulous papers on the test bed and pat hard the ELISA plate several times downward; then inject at least 0.35ml of diluted washing concentration for 1-2 minutes' soaking. Repeat this process as needed.

Automatic washing method: Washing by automatic plate washer: If there is an automatic plate washer, it should only be used in the test when you are quite familiar with its functions.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Human CRP were tested 20 times on one plate, respectively. 

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human CRP were tested on 3 different plates, 8 replicates in each plate. 

CV(%) = SD/meanX100

Intra-Assay: CV<10% 

Inter-Assay: CV<12%

Contact:Rebecca Yan

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Email: rebecca@spanbio.com