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Human Apolipoprotein A1 ELISA Kit(APO-A1) for Research Use
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Product Details:
Payment & Shipping Terms:
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Detailed Product Description
| Specificity: | 100% | Assay Type: | Quantitative |
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| Shelf Life: | Varies Depending On The Specific Kit, Typically 6-12 Months | Sample Volume: | 50-100 UL |
| Sensitivity: | Ranges From Picogram To Nanogram Levels Depending On The Specific Kit | Assay Principle: | Sandwich ELISA |
| Detection Method: | Colorimetric Or Chemiluminescent | Specificity: | High Specificity For The Target Protein Or Biomarker Being Measured |
| Sample Type: | Serum, Plasma, Cell Culture Supernatants, Urine, Tissue Homogenates, And Other Biological Fluids | Cross Reactivity: | No Cross-reactivity With Other Proteins |
| Species Reactivity: | Varies Depending On The Specific Kit, Typically Human, Mouse, Rat, Or Other Species | Sample Size: | Varies Depending On The Specific Kit, Typically 50-100 μL |
| Product Type: | ELISA Kit | Format: | 96-well Plate |
| Storage: | Store At -20°C | Kit Components: | Varies Depending On The Specific Kit, Typically Includes Pre-coated Microplate, Detection And Conjugate Antibodies, Standards, Buffers, And Substrates |
Human Apolipoprotein A1 ELISA Kit(APO-A1) for Research Use
Sample Types Validated:Serum, blood plasma,Saliva, Urine, and other related tissue Liquid.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Please read this insert completely prior to using the product. This kit is used to assay Human Apolipoprotein A1(apo-A1) on the basis of the Biotin double antibody sandwich technology. This Elisa kit takes one-step method with which solutions do not need diluting,because we simplifies the dilute process by our lab techniques. This kit is for research only and is not for use in diagnostic procedurs.
Intended Use
This kit is used to assay the Apolipoprotein A1(apo-A1)in the sample of Human’s serum, blood plasma and other related biological liquid.
Test principle
This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Human Apolipoprotein A1 (apo-A1). Add Apolipoprotein A1(apo-A1)to the wells, which are pre-coated with Apolipoprotein A1(apo-A1)monoclonal antibody and then incubate. After that, add anti apo-A1 antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Human Apolipoprotein A1 (apo-A1) are positively correlated.
Materials supplied in the Test Kit
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1 |
Standard solution(9.6mg/dl) |
0.5ml |
7 |
Chromogen solution A |
6ml |
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2 |
Standard dilution |
3ml |
8 |
Chromogen solution B |
6ml |
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3 |
Coated ELISA plate |
12-well * 8 tubes |
9 |
Stop solution |
6ml |
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4 |
Streptavidin-HRP |
6ml |
10 |
Instruction |
1 |
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5 |
Washing concentrate (30X) |
20ml |
11 |
Seal plate membrane |
2 |
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6 |
Anti apo-A1 antibodies labeled with biotin |
1ml |
12 |
Hermetic bag |
1 |
Materials required but not supplied
1. 37 ℃ incubator 2. Standard Enzyme reader
3. Precision pipettes and Disposable pipette tips 4. Distilled water
5. Disposable tubes for sample dilution 6. Absorbent paper
Rebecca Yan
Product Manager
Span Biotech Ltd.
Tel: +86(755)89589611
Cell Phone:+8618823462100(WhatsApp)
Web:www.spanbio.com
Tel: +86(755)89589611
Cell Phone:+8618823462100(WhatsApp)
Web:www.spanbio.com
Contact Details
SPAN BIOTECH LTD.
Contact Person: Ms. Anna Lee
Tel: +86-755-89589611
Fax: 86-755-89580096
Send your inquiry directly to us