Human Transferrin(TRF) ELISA Kit ,96T

Human Transferrin(TRF)ELISA Kit

Intended Use

This kit is used to assay the Transferrin(TRF)in the sample of Human’s serum, blood plasma and other related biological liquid.

Test principle

This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Human Transferrin (TRF). Add Transferrin(TRF)to the wells, which are pre-coated with Transferrin(TRF)monoclonal antibody and then incubate. After that, add anti TRF antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Human Transferrin (TRF) are positively correlated.

Materials supplied in the Test Kit

Materials required but not supplied

1. 37 ℃ incubator                              2. Standard Enzyme reader

3. Precision pipettes and Disposable pipette tips      4. Distilled water

5. Disposable tubes for sample dilution             6. Absorbent paper

Important Notes

1. Before opening the kit kept at the temperature of 2-8℃, it takes at least 30 minutes to increase naturally to room temperature. After breaking the seal of ELISA coated-plate, some of the stripes used should be kept in hermetic bag.

2.When adding samples, sample injector must be used for each time and should also be frequently checked for its precision to avoid individual error.

3. The instruction must be strictly followed while the reading of ELISA reader must be set as the standard of determining the experiment result.

4.Pipette tips and seal plate membrane in hand should not be used more than once in order to avoid cross contamination.

5.All samples, washing concentration and wastes of every kind should be disposed as infective agent.

6.Other reagents not needed must be packed or covered. Reagents of different batches must not be mixed and should be used before their respective validity dates.

7. Substrate B is sensitive to light and therefore should not be exposed to light for too long.