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THC Gold Rapid Screen Test
For the qualitative detection of THC in urine.
The THC Gold Rapid Screen Test is a rapid qualitative competitive binding immunoassay for determination of Marijuana in urine.
The THC Gold Rapid Screen Test is a fast and easy to read immunoassay with no need for instrument. The method employs a unique combination of monoclonal and polyclonal antibodies to specifically identify Marijuana in urine with a high degree of sensitivity. Marijuana is a hallucinogenic agent derived from the flowering portion of the hemp plant. The active ingredients in marijuana, THC and Cannabinol can be metabolized and excreted as 11-nor-D9-THC-9-COOH with a half-life of 24 hours. It can be detected for 1-5 days after use. Smoking is the primary method of use of marijuana/cannabis. Higher doses used by abusers produce central nervous system effects, altered mood and sensory perceptions, loss of coordination, impaired short-term memory, anxiety, paranoia, depression, confusion, hallucinations and increased heart rate. A tolerance to the cardiac and psychotropic effects can occur, and withdrawal syndrome produces restlessness, insomnia, anorexia and nausea. Marijuana is controlled substances. GC/MS or immunoassay method has been developed for the determination of 50ng/ml for Marijuana as set by the National Institute on Drug Abuse (NIDA).
The THC Gold Rapid Screen Test is a chromatographic absorbent device in which drug or drug metabolites in a sample compete with drug antigen immobilized on a porous membrane support for limited antibody sites. Labeled antibody-dye conjugate mixes with sample specimen and binds to the free drug presented in sample forming an antibody-antigen complex. This complex prevents the formation of pink color bands in the test zone immobilized antigen conjugate when the drug is presented in the sample urine above the detection levels (50ng/ml for marijuana). Unbound dye conjugate binds to the reagent in the control zone and produces a pink-rose color band, demonstrating that the reagents and device are functioning correctly.
A negative specimen produces two distinct color bands, one for the control in the “C” zone and one for marijuana in the “T” zone.
1. For in vitro diagnostic use only.
2. Do not use kit beyond the expiration date.
3. Urine specimens may be infectious; properly handle and dispose of all used reaction devices in biohazard container.
The kits should be stored at temperature 4-30°C the sealed pouch for the duration of the shelf life (18 months).
Collect a fresh urine sample in a clean, dry container, either plastic or glass, without any preservatives. Urine specimens may be refrigerated (2-8°C) and stored up to 48 hours before testing. Urine samples exhibiting visible precipitates should be filtered, centrifuged or allowed to settle. Use only clear aliquots for testing.
1. Bring the urine sample and test components to room temperature if refrigerated.
2. When ready to test, open the pouch at the notch and remove the test device. Place the test device on a clean, flat surface.
3. Fill the urine dropper with specimen. Holding the dropper vertically, dispense 2-3 drops (about 50-80 ml) of urine without air bubble into the sample well.
4. Read the result in 5-10 minutes.
1. Negative: Two pink color bands appear. This indicates THC in the specimen is below the detection sensitivity (50ng/ml).
2. Positive: Only one pink band for control appears. This indicates the THC in the specimen is above the detection sensitivity.
3. Invalid: The presence of a control line is necessary to validate test performance. If no band appears the test should be repeated with a new test device.
Note: There is no meaning attributed to color intensity for C and T lines.
Contact Person: Ms. Anna Lee