Avian Influenza Virus H5 Antigen Test

Avian Influenza Virus H5 Antigen Test

INTENDED USE

Avian Influenza Virus H5 Antigen Rapid Test is a sandwich lateral flow immunochromatographic assay for the qualitative detection of avian influenza virus H5 subtype (AIV H5 Ag) in avian secretions.

Assay Time: 5 - 10 min

Sample: Secretions

PRINCIPLE OF THE ASSAY

Avian Influenza Virus H5 Ag Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is enough AIV H5 antigen in the sample, a visible T band will appear. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Avian Influenza Virus H5 antigen in the sample.

KIT COMPINENT

• 10×foil pouches, each containing a cassette, pipette and desiccant
• 10×assay buffer tubes (1.0 mL each)
• 10×swab sticks
• Product Manual

TEST PROCEDURE

• Insert the swab stick into bird’s cloacae or dip the swab stick in bird’s eyes to collect the secretions. Please make the swab wet sufficiently.
• Insert the wet swab into the provided assay buffer tube. Agitate it to assure good sample extraction.
• Take out the cassette from the foil pouch and place it horizontally.
• Gradually drip 3 drops of sample extraction into the sample hole.
• Interpret the result in 5 - 10 minutes. Result after 10 minutes is only considered as reference.

INTERPRETATION OF RESULTS

Positive: The presence of both C band and T band, no matter T band is clear or vague.

Negative: Only clear C band appears.

Invalid: No colored band appears in C zone, no matter whether T band appears.

STORAGE

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

PRECAUTIONS

• For best results, please strictly adhere to these instructions.
• All reagents must be at room temperature before running the assay.
• Do not remove test cassette from its pouch until immediately before use.
• Do not reuse the test kit.
• Do not use the test beyond its expiration date marked on the foil pouch.
• The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

LIMITATION

Avian Influenza Virus H5 Antigen Rapid Test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available with veterinarian. For an accurate result, we suggest you to choose other method such as PCR for final determination in practice.

Span Biotech Ltd. is a research based company for rapid tests, with strong support from National Key Laboratory of Technology Projects of 10^th and 11^th Five-Year Plan and Faculty of Life Sciences of HuBei University. SpanBio also housed a R&D team that is developing gene recombination, cell cultivation and protein purification techniques. SpanBio pays strict attention on rapid tests for human being, animal diseases and food safety detection. It provides a number of customized services to professional distributors and partnering affiliates with excellent quality, competitive prices and super service.

Our mission:

• Always best of all and always pay attention to innovation.
• Special customized service tightly following customers’ requests.
• Integrated excellent quality, competitive prices and super service together.