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Human Interleukin 1β ELISA Kit

Human Interleukin 1β ELISA Kit

Human Interleukin 1β ELISA Kit

Product Details:

Place of Origin: CHINA
Brand Name: SPAN
Certification: Interleukin
Model Number: Interleukin

Payment & Shipping Terms:

Minimum Order Quantity: 1 KIT
Price: U$300/Kit
Packaging Details: 96T/Kit
Delivery Time: 5 days
Payment Terms: T/T, Western Union, , MoneyGram,Paypal
Supply Ability: Interleukin
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Detailed Product Description

Standard Curve Range: 20pg/L - 6000pg/L

Sensitivity: 10.07pg/L

Size: 96 wells

Storage: Store the reagents at 2-8°C. For long term storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

 

*This product is for research use only, not for use in diagnosis procedures. Its highly recommend to read this instruction entirely before the use.

 

PRECISION

 

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

 

INTENDED USE

This sandwich kit is for the accurate quantitative detection of human Interleukin 1β(also known as IL-1β)in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

ASSAY PRINCIPLE

This kit is a Enzyme-Linked Immunosorbent Assay (ELISA). IL-1β is added to the wells pre-coated with IL-1β monoclonal antibody. After cubation a biotin-conjugated anti-human IL-1β antibody is added and binds to human IL-1β. After incubation unbound biotin-conjugated anti-human IL-1β antibody is washed away during a washing step. Streptavidin-HRP is added and binds to the biotin-conjugated anti-human IL-1β antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human IL-1β. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

 

 

Contact Rebecca Yan

Skype: span.biotech

Email: rebecca@spanbio.com

Tel: +86(755)89589611

Fax: +86(755)89580096

Web:www.spanbio.com

www.spanbiotech.com

 

 

 

 

Contact Details
SPAN BIOTECH LTD.

Contact Person: Ms. Anna Lee

Tel: 86-755-89589611

Fax: 86-755-89580096

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