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Clenbuterol Elisa Kit- Food Safety
Clenbuterol ELISA Kit is a direct enzyme-linked immunosorbent assay for the quantitative detect the presence of Clenbuterol in samples of animal’s tissue, urine and feed.
Sensitivity: 0.05 ppb
Detection Limit: Animal’s tissue 0.2 ppb / 0.05 ppb
Urine 0.05 ppb
Feed 2.5 ppb
Recovery Rate: 90% ± 15% (pork liver, feed 80%±15%)
- 50 – 200 μL multichannel micropipette
- 50, 100 and 200 μL precision micropipette
- Microplate washer or squeeze bottle
- Centrifugal tubes
- Distilled water
- HCl, NaOH
PREPARATION OF WORKING SOLUTIONS
Washing buffer: dilute 10X concentrate with distilled water (i.e. 1 mL + 9 mL).
2% NaCl: dissolve 2.0±0.01 NaCL with 100 mL distilled water into a beaker
Animal’s tissue extract solution: mix 2% NaCl solution with 0.1M HCl at the volumetric ratio 4:1.
Standard solution, Enzyme-linked conjugate, substrate A, substrate B and stopping solution are ready-to-use.
Method 1 (dilution factor: 4):
Method 2 (dilution factor: 1):
Contact Person: Ms. Anna Lee