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Ractopamine Elisa Kit-Food Safety
INTENTED USE
Ractopamine ELISA Kit is an indirect enzyme-linked immunosorbent assay for the quantitative detect the presence of ractopamine in samples of animal’s tissue, urine and feed.
Sensitivity: 0.025 ppb
Detection Limit: Animal’s tissue 0.1 ppb / 0.025 ppb
Urine 0.025 ppb
Feed 1.25 ppb
Recovery Rate: 90% ± 15% (pork liver, feed 80% ± 15%)
KIT COMPONENT
- 50-200 μL multichannel micropipette
- 50, 100 and 200 μL precision micropipette
- Microplate washer or squeeze bottle
- Homogenizer
- Centrifuge
- Centrifugal tubes
- Distilled water
- HCl, NaOH
PREPARATION OF WORKING SOLUTIONS
Washing buffer: dilute 10X concentrate with distilled water (i.e. 1 mL + 9 mL).
1 M HCl: dilute 8.33mL concentrated hydrochloric acid with distilled water and make the constant volume to 100 mL, OR dilute 10X 1M HCl with distilled water (i.e. 1 mL + 9 mL).
Extract solution: mix 8g NaCl with 100mL 1M HCl .
Standard solution, Enzyme-linked conjugate, substrate A, substrate B and stopping solution are ready-to-use.
SAMPLE PREPARATION
1.Animal’s tissue
Method 1 (dilution factor: 4):
Method 2 (dilution factor: 1):
Take 50 μL for assay.
Contact Person: Ms. Anna Lee
Tel: +86-755-89589611
Fax: 86-755-89580096