Tetracycline Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Tetracycline residue in milk. Cut-off: 100 ppb Assay Time: 5 – 10 min Sample: Defatted milk
2. PRINCIPLE OF THE ASSAY
Tetracycline Rapid Test is based on competitive lateral flow immunochromatographic assay. The Tetracycline conjugate in the test zone will capture the immuno-gold (colloid gold- Tetracycline antibody conjugate), when there is very little dissociative Tetracycline in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if Tetracycline is present in concentration of 100 ppb and above which explains a positive result.
3. KIT COMPONENT
- 10×foil pouches each contain a cassette and desiccant
- 1×plastic canister containing 10 microwells and a desiccant
- 20×pipettes, 10×centrifugal tubes (1.5 mL)
- Product Manual
4. TEST PROCEDURE
- Bring 2-3 mL of the under tested milk to room temperature (20-30℃).
- Collect 1mL of milk sample into a centrifugal tube. Do centrifugation at 7000 rpm for 3-4 min to separate the fat component.
- Take out the microwells strip from the plastic canister. Take one well and tear off the film. Insert the pipette through the fat layer (5 mm below) to collect the defatted milk towards the mark on the pipette (200 μL) and transfer it into the well.
- Use another pipette to repeatedly suck and extrude the sample until all red reagents are completely dissolved. Wait for 1 min. NOTE: The sensitivity of the strip is positive correlated to the reaction time. It is suggested to wait for 1-2 min before running the assay.
- Take out the cassette from the foil pouch and place it horizontally.
- Suck the mixture in the well and gradually drip 3 drops into the assay sample hole “S”.
- Interpret the result in 5 - 10 minutes. Result after 10 minutes is only considered as reference.