Canine Heartworm Ag Test

Canine Heartworm Ag Test

1. INTENDED USE

Canine Heartworm Ag Rapid Test is a sandwich lateral flow immunochromatographic assay for the qualitative detection of Dirofilaria immitis (CHW Ag) in dog’s blood, serum or plasma.

Assay Time: 5-10 min

Sample: Serum, plasma or blood

1. PRINCIPLE OF THE ASSAY

Canine Heartworm Ag Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is enough CHW antigen in the sample, a visible T band will appear. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Dirofilaria immitis antigen in the sample.

1. KIT COMPONENT

• 10×foil pouches, each containing a cassette, pipette and desiccant
• 10×assay buffer tubes (0.5 mL each)
• 10×centrifugal tubes
• Product Manual

1. TEST PROCEDURE

• Take dog’s blood, serum or plasma as sample.
• Take out the cassette from the foil pouch and place it horizontally.
• Drip 1 drop of sample and immediately drip 2 drops of assay buffer into the sample hole. NOTE: It is suggested to use serum to increase the detection sensitivity.
• Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

1. INTERPRETATION OF RESULTS

Positive: The presence of both C band and T band, no matter T band is clear or vague.

Negative: Only clear C band appears.

Invalid: No colored band appears in C zone, no matter whether T band appears.

1. STORAGE

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

1. PRECAUTIONS

• For best results, please strictly adhere to these instructions.
• All reagents must be at room temperature before running the assay.
• Do not remove test cassette from its pouch until immediately before use.
• Do not reuse the test kit.
• Do not use the test beyond its expiration date marked on the foil pouch.
• The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

1. LIMITATION

Canine Heartworm Ag Rapid Test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, It is suggested to apply other method such as PCR for final determination in practice.

Span Biotech Ltd. is a research based company for rapid tests, with strong support from National Key Laboratory of Technology Projects of 10^th and 11^th Five-Year Plan and Faculty of Life Sciences of HuBei University. SpanBio also housed a R&D team that is developing gene recombination, cell cultivation and protein purification techniques. SpanBio pays strict attention on rapid tests for human being, animal diseases and food safety detection. It provides a number of customized services to professional distributors and partnering affiliates with excellent quality, competitive prices and super service.

Our mission:

• Always best of all and always pay attention to innovation.
• Special customized service tightly following customers’ requests.
• Integrated excellent quality, competitive prices and super service together.

Rebecca Yan

Product Manager
Span Biotech Ltd.
Tel: +86(755)89589611
Cell Phone:+8618823462100(WhatsApp)
Web:www.spanbio.com