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Home ProductsELISA Kit Tests

Human Vasoactive Intestinal Peptide(VIP) ELISA Kit

Human Vasoactive Intestinal Peptide(VIP) ELISA Kit

Human Vasoactive Intestinal Peptide(VIP) ELISA Kit
Human Vasoactive Intestinal Peptide(VIP) ELISA Kit

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Product Details:
Place of Origin: CHINA
Brand Name: SPAN
Certification: VIP
Model Number: VIP
Payment & Shipping Terms:
Minimum Order Quantity: 1 KIT
Price: U$300/Kit
Packaging Details: 96T/Kit
Delivery Time: 3-5 days
Payment Terms: T/T, Western Union, MoneyGram,Paypal
Supply Ability: VIP
Detailed Product Description

Intended Use

This kit is used to assay the Vasoactive Intestinal Peptide(VIP)in the sample of Human’s serum, blood plasma and other related biological liquid.


Test principle

This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Human Vasoactive Intestinal Peptide (VIP). Add Vasoactive Intestinal Peptide(VIP)to the wells, which are pre-coated with Vasoactive Intestinal Peptide(VIP)monoclonal antibody and then incubate. After that, add anti VIP antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Human Vasoactive Intestinal Peptide (VIP) are positively correlated.


Materials supplied in the Test Kit

1 Standard solution(640pg/ml) 0.5ml 7 Chromogen solution A 6ml
2 Standard dilution 3ml 8 Chromogen solution B 6ml
3 Coated ELISA plate 12-well * 8 tubes 9 Stop solution 6ml
4 Streptavidin-HRP 6ml 10 Instruction 1
5 Washing concentrate (30X) 20ml 11 Seal plate membrane 2
6 Anti VIP antibodies labeled with biotin 1ml 12 Hermetic bag 1


Materials required but not supplied

1. 37 ℃ incubator 2. Standard Enzyme reader

3. Precision pipettes and Disposable pipette tips 4. Distilled water

5. Disposable tubes for sample dilution 6. Absorbent paper


Important Notes

1. Before opening the kit kept at the temperature of 2-8℃, it takes at least 30 minutes to increase naturally to room temperature. After breaking the seal of ELISA coated-plate, some of the stripes used should be kept in hermetic bag.

2.When adding samples, sample injector must be used for each time and should also be frequently checked for its precision to avoid individual error.

3. The instruction must be strictly followed while the reading of ELISA reader must be set as the standard of determining the experiment result.

4.Pipette tips and seal plate membrane in hand should not be used more than once in order to avoid cross contamination.

5.All samples, washing concentration and wastes of every kind should be disposed as infective agent.

6.Other reagents not needed must be packed or covered. Reagents of different batches must not be mixed and should be used before their respective validity dates.

7. Substrate B is sensitive to light and therefore should not be exposed to light for too long.


Washing method

Manually washing method: Washing by hand: Shake off the liquids in the wells of the ELISA plate; Lay several bibulous papers on the test bed and pat hard the ELISA plate several times downward; then inject at least 0.35ml of diluted washing concentration for 1-2 minutes' soaking. Repeat this process as needed.

Automatic washing method: Washing by automatic plate washer: If there is an automatic plate washer, it should only be used in the test when you are quite familiar with its functions.



Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Human VIP were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human VIP were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%



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Tel: +86(755)89589611

Fax: +86(755)89580096

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Contact Details

Contact Person: Ms. Anna Lee

Tel: +86-755-89589611

Fax: 86-755-89580096

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