Human Interleukin 1β ELISA Test Kit

Standard Curve Range: 20pg/L - 6000pg/L

Sensitivity: 10.07pg/L

Size: 96 wells

Storage: Store the reagents at 2-8°C. For long term storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before the use.

PRECISION

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

INTENDED USE

This sandwich kit is for the accurate quantitative detection of human Interleukin 1β(also known as IL-1β)in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

ASSAY PRINCIPLE

This kit is a Enzyme-Linked Immunosorbent Assay (ELISA). IL-1β is added to the wells pre-coated with IL-1β monoclonal antibody. After cubation a biotin-conjugated anti-human IL-1β antibody is added and binds to human IL-1β. After incubation unbound biotin-conjugated anti-human IL-1β antibody is washed away during a washing step. Streptavidin-HRP is added and binds to the biotin-conjugated anti-human IL-1β antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human IL-1β. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.