Beta lactam + Tetracycline Combo Rapid Test

Beta lactam + Tetracycline Combo Rapid Test 2 in 1

1. INTENDED USE

Beta lactam + Tetracycline Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Beta lactam and Tetracycline residue in milk.

Assay Time:  5 - 10 min

Sample: milk

2. KIT COMPONENT

1.    12×plastic canister each containing 8 microwells and 8 strips

2.   96×pipettes

3.    Product Manual

3.TEST PROCEDURE

-Collect milk as testing sample.

-Take out the microwells and strips of necessary quantity from the plastic canister. Recover the milk and test kit to room temperature (20-25 °C). Do not heat the milk.

-Use pipette to transfer 200 μL of sample (a mark on the pipette) into the microwell.

-Repeatedly suck and extrude the sample until all red reagents are completely dissolved.

-Stand for 2 – 3 min and insert the “max” end of strip into the well.

-Read the result in 5 - 10 min. Result after 10 minutes is only considered as reference.

4. INTERPRETATION OF RESULTS

Positive: The T band does not appear.

Negative: The T band appears.

Invalid: No colored band appears in C zone.

5. SENSITIVITY AND SPECIFICITY

-Penicillin G 2 - 4 ppb, Ampicillin 10 - 15 ppb, Amoxicillin 4 - 5 ppb

-Oxacillin 10 - 15 ppb, Cloxacillin 10 - 15 ppb

-Cefacetril 125 ppb, Cefquinome 15 - 25 ppb

-Ceftiofur 50 ppb, Cefapirin 60 ppb.

-Tetracycline 100 ppb

-The results are negative when the rapid test is applied to detect 100 ppm of Chloramphenicol, Aminoglycosides, Macrolides and Sulfonamides.

6. STORAGE

All materials must be stored at 2 - 8°C. The test kit is stable through the expiration date (12 months) marked on the foil pouch. Do not store the test kit in direct sunlight.

7. PRECAUTIONS

-For best results, please strictly adhere to these instructions.

-All reagents must be at room temperature before running the assay.

-Do not remove rapid test from its pouch until immediately before use.

-The milk sample should be fresh and homogeneous liquid without caking and precipitation.

-Do not reuse the test strip.

-Do not use the test beyond its expiration date.

-Do not use water, powdered milk as negative control. Use confirmed negative fresh milk as control.

-The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

8.LIMITATION

Beta lactam + Tetracycline Rapid Test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. If you want a quantitative result, it is suggested to apply other method such as ELISA / HPLC in practice.