One-Step Cow Pregnancy Rapid Test - 6 specimen (Milk, Urine, Feces, Saliva ,Hair and Blood)

PRINCIPLE
Progesterone (P4) is a kind of steroid hormone. The concentration change of P4 in milk is one specific characteristic indicating the activities of cow ovary. It plays an important role in the process of maternal reproduction and maintaining pregnancy.
Therefore, it can be monitoring cow reproductive activity by detecting the concentration of P4. P4 concentration has different characteristic changes as the cow's stage of reproductive physiology, through to detect the progesterone levels can make an accurate diagnosis of cow in early pregnancy.
 
PRODUCT FEATURES
The strip can be used for diagnosis of cow early pregnancy, which is of high accuracy, easy operation, rapid and normal temperature storage, easy to carry, harmless to maternal and fetal safety.
THE SCOPE OF USE
The test is mainly suitable for dairy and beef cattle early pregnancy diagnosis, as well as to guide timely artificial insemination.
 
THE BEST USE DATE
Cow's estrus cycle is 21 days. There are individual differences in 18 to 24 days commonly, it is suggested to perform the test the 18^th days after mating. Perform the test once every day for 5 continuous days; even if one negative result appears during the five days, which indicates the cow is not pregnant and P4 is at the lowest level at this moment. In this case, it was suggested to have the dairy cow mated in time; that should improve the conception rate. If positive results show for all 5 days tests, it indicates the cow is in pregnancy, and it should get careful nursing and gestation management. Another test method is to closely observe the cow rutting performance from 18 days after mating, if it was in rutting performance the detection should do immediately; for those that have no heat after mating to do a test on the 24^th day.
 
SPECIMEN COLLECTION
1.Milk: cleaning teats before take the milk, abandon to the first three milk, then collect milk in the bottle, take 1 ml in the test tube, placed the centrifuge10,000 rpm for 10 minutes, in vitro milk sample was divided into three layers, using straws inserted in the underlying absorbing liquid cream and set aside.
2.Saliva: from the mouth collecting saliva for 3 to 5 ml placed the centrifuge 1,500-2,000 rpm for 5 minutes, take supernatant on standby.
3.Hair: from the hair root galley proof according to take 10 mg in the extracting pipe, add 3 ml anhydrous ethanol soak vortex shock 5 minutes after 2-3 hours, placed 3,000 rpm centrifuge for 5 minutes, drain on ethanol solution 1 ml into glass dish, in 70-80 ℃ oven dry reoccupy after 1 ml PBS dilution. Set aside.
4.Urine: can be used directly.
5.Blood:anticoagulant take 1 to 2 ml of centrifugal 2,000 rpm 5-10 minutes from the plasma standby, the anticoagulant let stand for 30 minutes to separate serum blood clots. Set aside.
6,Feces: Collect fresh stool sample from the rectum .Unscrew the top of the sample collection tube we provide, take out the sample collection stick, and collect the sample by dipping the stick into 3 different places of the stool sample. Then put the sample collection stick back in the sample collection tube and screw together tightly. (As picture below) Shake the sample collection tube several times and make it ready for use .Set aside. If the sample cannot be tested on the day of collection, store the stool sample at 4°C. Bring the specimen to room temperature before testing.
PRINCIPLE
Progesterone (P4) is a kind of steroid hormone. The concentration change of P4 in milk is one specific characteristic indicating the activities of cow ovary. It plays an important role in the process of maternal reproduction and maintaining pregnancy.
Therefore, it can be monitoring cow reproductive activity by detecting the concentration of P4. P4 concentration has different characteristic changes as the cow's stage of reproductive physiology, through to detect the progesterone levels can make an accurate diagnosis of cow in early pregnancy.
 
PRODUCT FEATURES
The strip can be used for diagnosis of cow early pregnancy, which is of high accuracy, easy operation, rapid and normal temperature storage, easy to carry, harmless to maternal and fetal safety.
THE SCOPE OF USE
The test is mainly suitable for dairy and beef cattle early pregnancy diagnosis, as well as to guide timely artificial insemination.
 
THE BEST USE DATE
Cow's estrus cycle is 21 days. There are individual differences in 18 to 24 days commonly, it is suggested to perform the test the 18^th days after mating. Perform the test once every day for 5 continuous days; even if one negative result appears during the five days, which indicates the cow is not pregnant and P4 is at the lowest level at this moment. In this case, it was suggested to have the dairy cow mated in time; that should improve the conception rate. If positive results show for all 5 days tests, it indicates the cow is in pregnancy, and it should get careful nursing and gestation management. Another test method is to closely observe the cow rutting performance from 18 days after mating, if it was in rutting performance the detection should do immediately; for those that have no heat after mating to do a test on the 24^th day.
 
SPECIMEN COLLECTION
1.Milk: cleaning teats before take the milk, abandon to the first three milk, then collect milk in the bottle, take 1 ml in the test tube, placed the centrifuge10,000 rpm for 10 minutes, in vitro milk sample was divided into three layers, using straws inserted in the underlying absorbing liquid cream and set aside.
2.Saliva: from the mouth collecting saliva for 3 to 5 ml placed the centrifuge 1,500-2,000 rpm for 5 minutes, take supernatant on standby.
3.Hair: from the hair root galley proof according to take 10 mg in the extracting pipe, add 3 ml anhydrous ethanol soak vortex shock 5 minutes after 2-3 hours, placed 3,000 rpm centrifuge for 5 minutes, drain on ethanol solution 1 ml into glass dish, in 70-80 ℃ oven dry reoccupy after 1 ml PBS dilution. Set aside.
4.Urine: can be used directly.
5.Blood:anticoagulant take 1 to 2 ml of centrifugal 2,000 rpm 5-10 minutes from the plasma standby, the anticoagulant let stand for 30 minutes to separate serum blood clots. Set aside.
6,Feces: Collect fresh stool sample from the rectum .Unscrew the top of the sample collection tube we provide, take out the sample collection stick, and collect the sample by dipping the stick into 3 different places of the stool sample. Then put the sample collection stick back in the sample collection tube and screw together tightly. (As picture below) Shake the sample collection tube several times and make it ready for use .Set aside. If the sample cannot be tested on the day of collection, store the stool sample at 4°C. Bring the specimen to room temperature before testing.
 
PRECAUTION
1. For single use only; do not reuse the test devices.
2. After open the packing, should be used immediately, Do not keep the strip or cassette in the air for a long time after unpacking.
3. While performing the test, make sure that the surface of sample should not exceed the “MAX” line of strip.
4. Do not touch the white membrane surface in the middle of test.
5. Do not use test kit after the expiry date.
 
WARNING
False negatives: the test performed on 90th day or later after mating appears negative result due to individual difference, which negative (a red line) should affirm with clinical examination, or using other methods for the testing.
 
 
VALIDITY
The period of the validity is 24 months.
 
 
STORAGE
Store as packaged in the sealed pouch under the room temperature. Avoid light.