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Chicken Tartrate-resistant Acid Phosphatase 5b(TRACP-5b) ELISA Kit

Chicken Tartrate-resistant Acid Phosphatase 5b(TRACP-5b) ELISA Kit

    • Chicken Tartrate-resistant Acid Phosphatase 5b(TRACP-5b) ELISA Kit
    • Chicken Tartrate-resistant Acid Phosphatase 5b(TRACP-5b) ELISA Kit
  • Chicken Tartrate-resistant Acid Phosphatase 5b(TRACP-5b) ELISA Kit

    Product Details:

    Place of Origin: CHINA
    Brand Name: SPAN
    Certification: TRACP-5b
    Model Number: TRACP-5b

    Payment & Shipping Terms:

    Minimum Order Quantity: 1 KIT
    Price: U$300/Kit
    Packaging Details: 96T/Kit
    Delivery Time: 5 days
    Payment Terms: T/T, Western Union,Paypal
    Supply Ability: TRACP-5b
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    Detailed Product Description

    Chicken Tartrate-resistant Acid Phosphatase 5b(TRACP-5b) ELISA Kit

     

    Sample Types Validated:Serum, blood plasma,Saliva, Urine, and other related tissue Liquid.

     

    FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

    Please read this insert completely prior to using the product. This kit is used to assay Chicken Tartrate-resistant Acid Phosphatase 5b(TRACP-5b) on the basis of the Biotin double antibody sandwich technology. This Elisa kit takes one-step method with which solutions do not need diluting,because we simplifies the dilute process by our lab techniques. This kit is for research only and is not for use in diagnostic procedures.

     

    Intended Use

    This kit is used to assay the Tartrate-resistant Acid Phosphatase 5b(TRACP-5b)in the sample of Chicken’s serum, blood plasma and other related biological liquid.

     

    Test principle

    This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Chicken Tartrate-resistant Acid Phosphatase 5b (TRACP-5b). Add Tartrate-resistant Acid Phosphatase 5b(TRACP-5b)to the wells, which are pre-coated with Tartrate-resistant Acid Phosphatase 5b(TRACP-5b)monoclonal antibody and then incubate. After that, add anti TRACP-5b antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Chicken Tartrate-resistant Acid Phosphatase 5b (TRACP-5b) are positively correlated.

     

    Materials supplied in the Test Kit

    1 Standard solution(24U/L) 0.5ml 7 Chromogen solution A 6ml
    2 Standard dilution 3ml 8 Chromogen solution B 6ml
    3 Coated ELISA plate 12-well * 8 tubes 9 Stop solution 6ml
    4 Streptavidin-HRP 6ml 10 Instruction 1
    5 Washing concentrate (30X) 20ml 11 Seal plate membrane 2
    6 Anti TRACP-5b antibodies labeled with biotin 1ml 12 Hermetic bag 1

    NoteIt is highly recommended to use the remaining reagents within 1 month provided this is with in the expiration date of the kit. For the expiration date of the kit, please refer to the label on the kit box. All components are stable until this expiration date.

    NoteTHE SAMPLE CAN'T BE DILUTED.

     

    Materials required but not supplied

    1. 37 ℃ incubator 2. Standard Enzyme reader 3. Precision pipettes and Disposable pipette tips 4. Distilled water

    5. Disposable tubes for sample dilution 6. Absorbent paper

     

    Important Notes

    1. Before opening the kit kept at the temperature of 2-8℃, it takes at least 30 minutes to increase naturally to room temperature. After breaking the seal of ELISA coated-plate, some of the stripes used should be kept in hermetic bag.

    2.When adding samples, sample injector must be used for each time and should also be frequently checked for its precision to avoid individual error.

    3. The instruction must be strictly followed while the reading of ELISA reader must be set as the standard of determining the experiment result.

    4.Pipette tips and seal plate membrane in hand should not be used more than once in order to avoid cross contamination.

    5.All samples, washing concentration and wastes of every kind should be disposed as infective agent.

    6.Other reagents not needed must be packed or covered. Reagents of different batches must not be mixed and should be used before their respective validity dates.

    7. Substrate B is sensitive to light and therefore should not be exposed to light for too long.

     

    Washing method

    Manually washing method: Washing by hand: Shake off the liquids in the wells of the ELISA plate; Lay several bibulous papers on the test bed and pat hard the ELISA plate several times downward; then inject at least 0.35ml of diluted washing concentration for 1-2 minutes' soaking. Repeat this process as needed.

    Automatic washing method: Washing by automatic plate washer: If there is an automatic plate washer, it should only be used in the test when you are quite familiar with its functions.

     

    Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chicken TRACP-5b were tested 20 times on one plate, respectively.

    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chicken TRACP-5b were tested on 3 different plates, 8 replicates in each plate.

    CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

     

    Specimen requirements

    1.Samples containing NaN3 must not be tested as it inhibits the activity of Horse Radish Peroxidase (HRP).

    2.After collecting the sample, extraction should be immediately carried out in accordance with related documents. After extraction, experiment should be conducted immediately as well. Otherwise, keep the sample at -20℃. Avoid repeated freeze-thaw cycles.

    3.Serum: Allow the serum to clot for 10-20 minutes at room temperature. Centrifuge (at 2000-3000 RPM) for 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.

    4.Blood plasma: In accordance with the requirements of sample collection, EDTA or sodium citrate should be used as anti coagulation. Add EDTA or sodium citrate and mix them for 10-20 minutes. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.

    5.Urine: Collect by sterile tube. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

    6.Cell culture supernatant: Collect by sterile tubes when examining secrete components. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (PH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.

    7.Tissue sample: Incise sample and weigh up. Add a certain amount of PBS (PH 7.4). Freeze with liquid nitrogen immediately for later use. Thaw the sample and keep it at 2-8℃. Add a certain amount of PBS (PH 7.4) and then homogenize the sample thoroughly by hand or homogenizer. Centrifuge (at 2000-3000 RPM) for approximately 20 minutes. Collect the supernatants carefully. Aliquot and keep one for examination and freeze the others for later use.

     

    Contact Rebecca Yan

    Skype: span.biotech

    Email: rebecca@spanbio.com

    Tel: +86(755)89589611

    Fax: +86(755)89580096

    Web:www.spanbio.com

    www.spanbiotech.com

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